Ube2t Antikörper

Produktnummer ABIN7120563

Dieser Anti-Ube2t Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von Ube2t in WB, ELISA und IP. Geeignet für Human, Maus und Ratte.

Kurzübersicht für Ube2t Antikörper (ABIN7120563)

Target: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ube2t Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-Ube2t Antikörper

Verwendungszweck: UBE2T antibody

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: ubiquitin-conjugating enzyme E2T(putative)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:200-1:2000, IP: 1:200-1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu Ube2t

Target: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Andere Bezeichnung: UBE2T

Hintergrund:

Synonyms: Ubiquitin-conjugating enzyme E2 T|Cell proliferation-inducing gene 50 protein|E2 ubiquitin-conjugating enzyme T|Ubiquitin carrier protein T|Ubiquitin-protein ligase T|UBE2T

Background: The ubiquitin(Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic(UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs(TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.

Molekulargewicht: 24 kDa

Gen-ID: 28089

UniProt: Q9NPD8

Pathways: Chromatin Binding